Figure 1: Schematic representation of the continuous FFE apparatus coupled off-line to RP-HPLC. Hydroxypropyl methyl cellulose (HPMC) stock solution, 2% (wt/vol; ∼86 K nominal molecular weight; ...
Given the nature of biomolecules, such as amino acids and peptides, many HPLC reversed phase bio-separations often require ion-pairing reagents, such as Trifluoroacetic acid (TFA), in combination with ...
Evaluation of: Stephanowitz H, Lange S, Lang D et al. Improved two-dimensional reversed phase–reversed phase LC-MS/MS approach for identification of peptide–protein interactions. J. Proteome Res. 11(2 ...
Any analytical method must strike a balance between its intrinsic scientific potential its capacity for robustness and reliability, particularly in regulated industries. In the case of HPLC, this ...
The term liquid chromatography (LC) refers to a class of physicochemical methods which are used to separate and quantify compounds in a sample. LC is made up of a liquid mobile phase, which carries ...
Announcing a new article publication for BIO Integration journal. Ellagic acid (EA) is a natural polyphenolic compound recognized for bioactive and pharmacologic properties that is found abundantly in ...
An interview with Dr. Michael Dong, about his upcoming talk at Pittcon. Why are stability-indicating methods important in pharmaceutical analysis? The stability-indicating assay is perhaps the single ...
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