Here we describe the generation of a double-fluorescent Cre/loxP indicator system. This protocol involves (i) all cloning steps to generate the plasmid vector (3–5 months); (ii) a guide to prepare ...

Foamy viruses (FVs) or spumaviruses are retroviruses that have been developed as vectors, but their integration patterns have not been described. We have performed a large-scale analysis of FV ...

Scientists have eliminated the need for a viral vector in the stem cell reprogramming process In a report in Science, they showed the ability to reprogram adult cells into iPS cells without viral ...