To generate a tissue specific knockout mouse, researchers breed a mouse bearing a Cre transgene under a tissue- or cell-type specific or inducible promoter (A) with a homozygous floxed mouse (B). The ...

To discover the function of a gene researchers turn it off and observe the consequences. Often genes have multiple functions that differ depending on a tissue and age. Some genes are essential to ...

A new editorial paper was published in Oncoscience (Volume 10) on November 14, 2023, entitled, "Be mindful of potential pitfalls when using the Cre-LoxP system in cancer research." In this new ...

We developed a cell division–activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and ...

Cre-loxP, a powerful tool for preclinical cancer research, possesses intrinsic pitfalls that can alter result accuracy, according to an editorial paper in Oncoscience. Cancer and other diseases could ...

Scientists of the National Center for Cardiovascular Research (CNIC) led by Rui Benedito have developed a new genetic tool (iSuRe-Cre) that provides certainty in Cre-inducible genetic modifications, a ...

We have created a stable transgenic rag2-EGFP-mMyc zebrafish line that develops GFP-labeled T cell acute lymphoblastic leukemia (T-ALL), allowing visualization of the onset and spread of this disease.