The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...
The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...
Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...
Polymerase Chain Reaction (PCR): the technique we all love to hate. PCR is immensely useful, but notoriously finicky. One of the biggest (and most important) variables that determines the success or ...
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